Tools for Single Cell Genomics


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Documentation for package ‘Seurat’ version 2.3.1

Help Pages

A B C D E F G H I J K L M N O P R S T U V W

-- A --

AddImputedScore Calculate imputed expression values
addImputedScore Deprecated function(s) in the Seurat package
AddMetaData Add Metadata
addMetaData Deprecated function(s) in the Seurat package
AddModuleScore Calculate module scores for gene expression programs in single cells
AddSamples Add samples into existing Seurat object.
AddSmoothedScore Calculate smoothed expression values
addSmoothedScore Deprecated function(s) in the Seurat package
add_samples Deprecated function(s) in the Seurat package
AlignSubspace Align subspaces using dynamic time warping (DTW)
as.seurat Convert Seurat objects to other classes and vice versa
as.seurat.SingleCellExperiment Convert Seurat objects to other classes and vice versa
as.SingleCellExperiment Convert Seurat objects to other classes and vice versa
as.SingleCellExperiment.seurat Convert Seurat objects to other classes and vice versa
AssessNodes Assess Internal Nodes
AssessSplit Assess Cluster Split
AugmentPlot Augments ggplot2 scatterplot with a PNG image.
average.expression Deprecated function(s) in the Seurat package
average.pca Deprecated function(s) in the Seurat package
AverageDetectionRate Probability of detection by identity class
AverageExpression Averaged gene expression by identity class
AveragePCA Average PCA scores by identity class

-- B --

batch.gene Deprecated function(s) in the Seurat package
BlackAndWhite A black and white color palette
BuildClusterTree Phylogenetic Analysis of Identity Classes
buildClusterTree Deprecated function(s) in the Seurat package
BuildRFClassifier Build Random Forest Classifier
BuildSNN SNN Graph Construction

-- C --

calc.insitu Deprecated function(s) in the Seurat package
CalcAlignmentMetric Calculate an alignment score
calcNoiseModels Deprecated function(s) in the Seurat package
CalcVarExpRatio Calculate the ratio of variance explained by ICA or PCA to CCA
calinskiPlot Deprecated function(s) in the Seurat package
CaseMatch Match the case of character vectors
cc.genes Cell cycle genes
cell.cor.matrix Deprecated function(s) in the Seurat package
CellCycleScoring Score cell cycle phases
CellPlot Cell-cell scatter plot
cellPlot Deprecated function(s) in the Seurat package
ClassifyCells Classify New Data
cluster.alpha Deprecated function(s) in the Seurat package
CollapseSpeciesExpressionMatrix Slim down a multi-species expression matrix, when only one species is primarily of interenst.
ColorTSNESplit Color tSNE Plot Based on Split
CombineIdent Sets identity class information to be a combination of two object attributes
Convert Convert Seurat objects to other classes and vice versa
Convert.anndata.base.AnnData Convert Seurat objects to other classes and vice versa
Convert.seurat Convert Seurat objects to other classes and vice versa
Convert.SingleCellExperiment Convert Seurat objects to other classes and vice versa
CreateSeuratObject Initialize and setup the Seurat object
CustomDistance Run a custom distance function on an input data matrix
CustomPalette Create a custom color palette

-- D --

DarkTheme Dark Theme
DBClustDimension Perform spectral density clustering on single cells
DBclust_dimension Deprecated function(s) in the Seurat package
DESeq2DETest Differential expression using DESeq2
diffExp.test Deprecated function(s) in the Seurat package
DiffExpTest Likelihood ratio test for zero-inflated data
DiffTTest Differential expression testing using Student's t-test
DimElbowPlot Quickly Pick Relevant Dimensions
DimHeatmap Dimensional reduction heatmap
DimPlot Dimensional reduction plot
DimTopCells Find cells with highest scores for a given dimensional reduction technique
DimTopGenes Find genes with highest scores for a given dimensional reduction technique
DMEmbed Diffusion Maps Cell Embeddings Accessor Function
DMPlot Plot Diffusion map
DoHeatmap Gene expression heatmap
doHeatMap Deprecated function(s) in the Seurat package
DoKMeans K-Means Clustering
doKMeans Deprecated function(s) in the Seurat package
dot.plot Deprecated function(s) in the Seurat package
DotPlot Dot plot visualization
DotPlotOld Old Dot plot visualization (pre-ggplot implementation) Intuitive way of visualizing how gene expression changes across different identity classes (clusters). The size of the dot encodes the percentage of cells within a class, while the color encodes the AverageExpression level of 'expressing' cells (green is high).

-- E --

ExpMean Calculate the mean of logged values
ExpSD Calculate the standard deviation of logged values
ExpVar Calculate the variance of logged values
ExtractField Extract delimiter information from a string.

-- F --

FastWhichCells FastWhichCells Identify cells matching certain criteria (limited to character values)
feature.heatmap Deprecated function(s) in the Seurat package
feature.plot Deprecated function(s) in the Seurat package
feature.plot.keynote Deprecated function(s) in the Seurat package
FeatureHeatmap Vizualization of multiple features
FeatureLocator Feature Locator
FeaturePlot Visualize 'features' on a dimensional reduction plot
fetch.data Deprecated function(s) in the Seurat package
FetchData Access cellular data
FilterCells Return a subset of the Seurat object
find.markers Deprecated function(s) in the Seurat package
find.markers.node Deprecated function(s) in the Seurat package
FindAllMarkers Gene expression markers for all identity classes
FindAllMarkersNode Find all markers for a node
FindClusters Cluster Determination
FindConservedMarkers Finds markers that are conserved between the two groups
FindMarkers Gene expression markers of identity classes
FindMarkersNode Gene expression markers of identity classes defined by a phylogenetic clade
FindVariableGenes Identify variable genes
find_all_markers Deprecated function(s) in the Seurat package
fit.gene.k Deprecated function(s) in the Seurat package
fit.gene.mix Deprecated function(s) in the Seurat package
FitGeneK Build mixture models of gene expression

-- G --

gene.cor.matrix Deprecated function(s) in the Seurat package
GenePlot Scatter plot of single cell data
genePlot Deprecated function(s) in the Seurat package
genes.in.cluster Deprecated function(s) in the Seurat package
geneScorePlot Deprecated function(s) in the Seurat package
GenesInCluster GenesInCluster
get.centroids Deprecated function(s) in the Seurat package
GetAssayData Accessor function for multimodal data
GetCellEmbeddings Dimensional Reduction Cell Embeddings Accessor Function
GetCentroids Get cell centroids
GetClusters Get Cluster Assignments
GetDimReduction Dimensional Reduction Accessor Function
GetGeneLoadings Dimensional Reduction Gene Loadings Accessor Function
getNewScore Deprecated function(s) in the Seurat package

-- H --

HeatmapNode Deprecated function(s) in the Seurat package
HoverLocator Hover Locator
HTODemux Demultiplex samples based on data from cell 'hashing'

-- I --

ICA Deprecated function(s) in the Seurat package
ica Deprecated function(s) in the Seurat package
ica.plot Deprecated function(s) in the Seurat package
ICAEmbed ICA Cell Embeddings Accessor Function
ICALoad ICA Gene Loadings Accessor Function
ICAPlot Plot ICA map
ICHeatmap Independent component heatmap
icHeatmap Deprecated function(s) in the Seurat package
ICTopCells Find cells with highest ICA scores
ICTopGenes Find genes with highest ICA scores
icTopGenes Deprecated function(s) in the Seurat package
initial.mapping Deprecated function(s) in the Seurat package
InitialMapping Infer spatial origins for single cells

-- J --

jackRandom Deprecated function(s) in the Seurat package
JackStraw Determine statistical significance of PCA scores.
jackStraw Deprecated function(s) in the Seurat package
jackStraw.permutation.test Deprecated function(s) in the Seurat package
jackStrawFull Deprecated function(s) in the Seurat package
jackStrawMC Deprecated function(s) in the Seurat package
JackStrawPlot JackStraw Plot
jackStrawPlot Deprecated function(s) in the Seurat package
JoyPlot Deprecated function(s) in the Seurat package

-- K --

KClustDimension Perform spectral k-means clustering on single cells
Kclust_dimension Deprecated function(s) in the Seurat package
KMeansHeatmap Plot k-means clusters
kMeansHeatmap Deprecated function(s) in the Seurat package

-- L --

LogNormalize Normalize raw data
LogVMR Calculate the variance to mean ratio of logged values

-- M --

MakeSparse Make object sparse
map.cell Deprecated function(s) in the Seurat package
marker.test Deprecated function(s) in the Seurat package
MarkerTest ROC-based marker discovery
MASTDETest Differential expression using MAST
MatrixRowShuffle Independently shuffle values within each row of a matrix
MeanVarPlot Deprecated function(s) in the Seurat package
MergeNode Merge childen of a node
MergeSeurat Merge Seurat Objects
MetageneBicorPlot Plot CC bicor saturation plot
MinMax Apply a ceiling and floor to all values in a matrix
minusc Deprecated function(s) in the Seurat package
minusr Deprecated function(s) in the Seurat package

-- N --

NegBinomDETest Negative binomial test for UMI-count based data
NegBinomRegDETest Negative binomial test for UMI-count based data (regularized version)
NormalizeData Normalize Assay Data
NumberClusters Convert the cluster labels to a numeric representation

-- O --

OldDoHeatmap Gene expression heatmap

-- P --

pbmc_small A small example version of the PBMC dataset
PCA Deprecated function(s) in the Seurat package
pca Deprecated function(s) in the Seurat package
pca.plot Deprecated function(s) in the Seurat package
pca.sig.genes Deprecated function(s) in the Seurat package
PCAEmbed PCA Cell Embeddings Accessor Function
PCALoad PCA Gene Loadings Accessor Function
PCAPlot Plot PCA map
PCASigGenes Significant genes from a PCA
PCElbowPlot Quickly Pick Relevant PCs
PCHeatmap Principal component heatmap
pcHeatmap Deprecated function(s) in the Seurat package
PCTopCells Find cells with highest PCA scores
pcTopCells Deprecated function(s) in the Seurat package
PCTopGenes Find genes with highest PCA scores
pcTopGenes Deprecated function(s) in the Seurat package
PlotClusterTree Plot phylogenetic tree
plotClusterTree Deprecated function(s) in the Seurat package
plotNoiseModel Deprecated function(s) in the Seurat package
PoissonDETest Poisson test for UMI-count based data
posterior.plot Deprecated function(s) in the Seurat package
PrintAlignSubspaceParams Print AlignSubspace Calculation Parameters
PrintCalcParams Print the calculation
PrintCalcVarExpRatioParams Print Parameters Associated with CalcVarExpRatio
PrintCCAParams Print CCA Calculation Parameters
PrintDim Print the results of a dimensional reduction analysis
PrintDMParams Print Diffusion Map Calculation Parameters
PrintFindClustersParams Print FindClusters Calculation Parameters
PrintICA Print the results of a ICA analysis
PrintICAParams Print ICA Calculation Parameters
PrintPCA Print the results of a PCA analysis
PrintPCAParams Print PCA Calculation Parameters
PrintSNNParams Print SNN Construction Calculation Parameters
PrintTSNEParams Print TSNE Calculation Parameters
project.pca Deprecated function(s) in the Seurat package
project.samples Deprecated function(s) in the Seurat package
ProjectDim Project Dimensional reduction onto full dataset
ProjectPCA Project Principal Components Analysis onto full dataset
PurpleAndYellow A purple and yellow color palette

-- R --

Read10X Load in data from 10X
refined.mapping Deprecated function(s) in the Seurat package
RefinedMapping Quantitative refinement of spatial inferences
RegressOut Deprecated function(s) in the Seurat package
regulatorScore Deprecated function(s) in the Seurat package
RemoveFromTable Remove data from a table
removePC Deprecated function(s) in the Seurat package
rename.ident Deprecated function(s) in the Seurat package
RenameIdent Rename one identity class to another
ReorderIdent Reorder identity classes
RidgePlot Single cell ridge plot
RunCCA Perform Canonical Correlation Analysis
RunDiffusion Run diffusion map
RunICA Run Independent Component Analysis on gene expression
RunMultiCCA Perform Canonical Correlation Analysis with more than two groups
RunPCA Run Principal Component Analysis on gene expression using IRLBA
RunTSNE Run t-distributed Stochastic Neighbor Embedding
RunUMAP Run UMAP
run_diffusion Deprecated function(s) in the Seurat package
run_tsne Deprecated function(s) in the Seurat package

-- S --

SampleUMI Sample UMI
SaveClusters Save cluster assignments to a TSV file
ScaleData Scale and center the data.
ScaleDataR Old R based implementation of ScaleData. Scales and centers the data
set.all.ident Deprecated function(s) in the Seurat package
set.ident Deprecated function(s) in the Seurat package
SetAllIdent Switch identity class definition to another variable
SetAssayData Assay Data Mutator Function
SetClusters Set Cluster Assignments
SetDimReduction Dimensional Reduction Mutator Function
SetIdent Set identity class information
seurat The Seurat Class
seurat-class The Seurat Class
Seurat-deprecated Deprecated function(s) in the Seurat package
show show method for seurat
show-method show method for seurat
Shuffle Shuffle a vector
spatial.de Deprecated function(s) in the Seurat package
SplitDotPlotGG Split Dot plot visualization
SplitObject Splits object into a list of subsetted objects.
StashIdent Set identity class information
SubsetByPredicate Return a subset of the Seurat object.
subsetCells Deprecated function(s) in the Seurat package
SubsetColumn Return a subset of columns for a matrix or data frame
SubsetData Return a subset of the Seurat object
subsetData Deprecated function(s) in the Seurat package
SubsetRow Return a subset of rows for a matrix or data frame

-- T --

tnse.plot Deprecated function(s) in the Seurat package
tobit.test Deprecated function(s) in the Seurat package
TobitTest Differential expression testing using Tobit models
TransferIdent Transfer identity class information (or meta data) from one object to another
tsne.plot Deprecated function(s) in the Seurat package
TSNEPlot Plot tSNE map

-- U --

UpdateSeuratObject Update old Seurat object to accomodate new features

-- V --

ValidateClusters Cluster Validation
ValidateSpecificClusters Specific Cluster Validation
VariableGenePlot View variable genes
viz.ica Deprecated function(s) in the Seurat package
viz.pca Deprecated function(s) in the Seurat package
VizClassification Deprecated function(s) in the Seurat package
VizDimReduction Visualize Dimensional Reduction genes
VizICA Visualize ICA genes
VizPCA Visualize PCA genes
VlnPlot Single cell violin plot
vlnPlot Deprecated function(s) in the Seurat package

-- W --

which.cells Deprecated function(s) in the Seurat package
WhichCells Identify cells matching certain criteria
WilcoxDETest Differential expression using Wilcoxon Rank Sum
writ.table Deprecated function(s) in the Seurat package