Tools for counting and visualising mutations in a target location


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Documentation for package ‘CrispRVariants’ version 1.6.0

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abifToFastq Read a file in ab1 (Sanger) format and convert to fastq
addClipped Extrapolates mapping location from clipped, aligned reads
addClipped-method Extrapolates mapping location from clipped, aligned reads
addCodonFrame Internal CrispRVariants function for indicating codon frame on an alignment tile plot
alns Get alignments
alns-method Get alignments
annotateGenePlot Plots and annotates transcripts
arrangePlots Arrange plots for plotVariants:CrisprSet
barplotAlleleFreqs Plots barplots of the spectrum of variants for a sample set
barplotAlleleFreqs-method Plots barplots of the spectrum of variants for a sample set
collapsePairs Internal CrispRVariants function for collapsing pairs with concordant indels
consensusSeqs Get consensus sequences for variant alleles
consensusSeqs-method Get consensus sequences for variant alleles
countDeletions Count the number of reads containing an insertion or deletion
countDeletions-method Count the number of reads containing an insertion or deletion
countIndels Count the number of reads containing an insertion or deletion
countIndels-method Count the number of reads containing an insertion or deletion
countInsertions Count the number of reads containing an insertion or deletion
countInsertions-method Count the number of reads containing an insertion or deletion
CrisprRun CrisprRun class
CrisprRun-class CrisprRun class
CrisprSet CrisprSet class
CrisprSet-class CrisprSet class
dispatchDots dispatchDots
excludeFromBam Removes reads from a bam file
findChimeras Find chimeric reads
findSNVs Find frequent SNVs
findSNVs-method Find frequent SNVs
getChimeras Get chimeric alignments
getChimeras-method Get chimeric alignments
gol Variant sequences from golden clutch 1 (Burger et al)
gol_clutch1 Variant sequences from golden clutch 1 (Burger et al)
indelPercent Count the number of reads containing an insertion or deletion
indelPercent-method Count the number of reads containing an insertion or deletion
makeAlignmentTilePlot Internal CrispRVariants function for creating the plotAlignments background
mergeChimeras mergeChimeras
mergeCrisprSets Merge two CrisprSets
mergeCrisprSets-method Merge two CrisprSets
mutationEfficiency Get mutation efficiency
mutationEfficiency-method Get mutation efficiency
narrowAlignments Narrow a set of aligned reads to a target region
narrowAlignments-method Narrow a set of aligned reads to a target region
plotAlignments Plot alignments with respect to a reference sequence
plotAlignments-method Plot alignments with respect to a reference sequence
plotChimeras Display a dot plot of chimeric alignments
plotFreqHeatmap Plot a table of counts with colours indicating frequency
plotFreqHeatmap-method Plot a table of counts with colours indicating frequency
plotVariants Plot alignments, frequencies and location of target sequence
plotVariants-method Plot alignments, frequencies and location of target sequence
rcAlns Internal CrispRVariants function for determining read orientation
readsByPCRPrimer Finds overlaps between aligned reads and PCR primers
readsByPCRPrimer-method Finds overlaps between aligned reads and PCR primers
readsToTarget Trims reads to a target region.
readsToTarget-method Trims reads to a target region.
readsToTargets Trims reads to a target region.
readsToTargets-method Trims reads to a target region.
readTargetBam Internal CrispRVariants function for reading and filtering a bam file
refFromAlns refFromAlns
refFromAlns-method refFromAlns
reverseCigar Reverses the order of operations in a cigar string
rmMultiPCRChimera Remove chimeric reads overlapping multiple primers
rmMultiPCRChimera-method Remove chimeric reads overlapping multiple primers
seqsToAln Creates a text alignment from a set of cigar strings
seqsToPartialAln seqsToPartialAln
setDNATileColours Sets colours for plotting aligned DNA sequences.
transformAlnsToLong Transform data for plotting
variantCounts Get variant counts
variantCounts-method Get variant counts
writeFastq Append a sequence to a fastq file