The 'PD RESULT' window is displayed automatically after finishing the probe target searching or manually by pressing the 'RESULT' button of the 'PROBE DESIGN' window.
The parameters defined by in the 'PROBE DESIGN' window are listed in the first few lines of the 'PD RESULT' window.
Description of single display columns:
Target the target sequence of the designed probe
le length of designed probe
apos absolute 5'-position of the target sites
(most often targeted position)
ecol ecoli-relative position
qual a probe quality indicator (see below)
grps groupsize (number of perfect hits in target-species)
G+C G+C content
temp predicted dissociation temperature (4GC+2AT)
Probe the designed probe sequence
The numbers of the next columns (from left to right) indicates how many non-target species would be detected by this probe if the temperature would be gradually lowered ('_' means no hits).
The columns do only represent virtual temperature shifts and cannot be assigned to degree centigrade!
To write the results to an ascii file press the 'SAVE' button to display the 'SELECTION BOX' window.
The 'Auto match' toggle has the same meaning as in ´Probe Match´.
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